ASSESSING FERTILITY:EXAMINATION OF FRESH UNINCUBATED EGGS (BREAKING OUT)..BLASTODERM :STEP 0NE

BREAKING OUT FRESH UN-INCUBATED EGGS

after fertilization,the eggs spends about a day travelling down the oviduct.during this time the number of cells in the blastoderm increases to about 60000.the characteristic organization of these cells just under the yolk sac membrane make it possible,with practice,to distinguish between an infertile blastodisc and a fertile blastoderm when breaking out fresh un incubated eggs.
the onfertile blastodisc is a small dense white area about two mm across. the white area is usually of irragullar of an irragullar shape and is never perfectly round.it is surrounded by a clear,roughly circular area upto four mm in diameter which appears to be filled with bubbles which are globules of yolk.

infertile egg (naked eye)

Magnified blastodisc of a fresh un incubated infertile egg..

The fertile blastoderm,by contrast,is largest (4-5 mm diameter)than the dense white area of the infertile blastodisc and is always uniformly round the usual form is that of a white ring or doughnut with a clear center .insome eggs there may be a small white spot in the center of the ring,occasionally eggs are seen which were laid with the blastoderm at an earlier stage of development when it will appear as a solid white,perfectly round disc.

 A fertile unincubated fertile egg as it appears to the naked eye..

Magnified blastoderm of a fresh unincubated fertile egg showing organized ring structure